mitochondria membrane 70  (Cell Signaling Technology Inc)

Journal: Journal of Sport and Health Science

Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

doi: 10.1016/j.jshs.2025.101095

Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

Article Snippet: Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: Schematic diagram of the preparation and anticancer mechanism of M1@CAT-Ce6-Rh2 Lips. (A) Schematic illustration of the preparation procedure for M1 macrophage membrane-camouflaged self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). Briefly, Nanoparticles were prepared using the anticancer drug Rh2 as the membrane material to encapsulate the photosensitizer Ce6 and CAT, which were then coated with lipopolysaccharide (LPS)-stimulated M1 macrophage membrane to enhance uptake by tumor cells. (B) Schematic depiction of the in vivo fate and mechanism of antineoplastic activity of M1@CAT-Ce6-Rh2 Lips upon intravenous administration. These biomimetic nanoparticles possess prolonged systemic circulation duration and preferential enrichment within tumor regions through the enhanced permeability and retention (EPR) effect. Following internalization into cancer cells, each functional component exerts its unique biological activity, synergistically triggering ferroptosis in cancer cells and ultimately exerting potent antitumor efficacy.

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: Membrane, In Vivo, Activity Assay, Permeability, Functional Assay

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: Physicochemical characterization of biomimetic self-oxygenating nanoparticles (M1@CAT-Ce6-Rh2 Lips). (A) Schematic illustration of the preparation of biomimetic nanoparticles; (B) UV-Vis absorption spectrum of M1@CAT-Ce6-Rh2 Lips; (C) FT-IR spectrum of Ce6-Rh2 Lips; (D) Representative particle size and ζ-potential profiles of M1@CAT-Ce6-Rh2 Lips; (E) Quantitative analysis of particle size for different formulations (n = 3); (F) Quantitative analysis of PDI values (n = 3); (G) Quantitative analysis of ζ-potential (n = 3); (H) Encapsulation efficiency of different formulations (n = 3); (I) TEM image of M1@CAT-Ce6-Rh2 Lips, scale bar = 50 nm; (J) SDS-PAGE analysis of protein profiles for CAT-Ce6-Rh2 Lips, M1@CAT-Ce6-Rh2 Lips, and M1 macrophage membrane; (K) Western blot analysis of integrin α4 and integrin β1 expression in M1 macrophages, M1 macrophage membrane, and M1@CAT-Ce6-Rh2 Lips; (L – O) Stability of different formulations after storage at 4 °C for 28 days (L, M) or incubation in serum for 48 h (N, O). Changes in encapsulation efficiency (L, N) and particle size (M, O) were quantitatively analyzed. Data are shown as mean ± SD (n = 3); (P) In vitro release profiles of Ce6 from various formulations; (Q) Time-dependent relative catalytic activities of free CAT, CAT-Ce6-Rh2 Lips, and M1@CAT-Ce6-Rh2 Lips upon proteinase K treatment; (R) Hemolytic activity of different formulations, where “−” and “+” indicate negative and positive controls, respectively.

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: Encapsulation, SDS Page, Membrane, Western Blot, Expressing, Incubation, In Vitro, Activity Assay

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: In vitro targeting evaluation of biomimetic self-oxygenating M1@CAT-Ce6-Rh2 Lips. (A) Flow cytometric analysis of the uptake of different formulations by LLC cells; (B) Quantitative analysis of relative cellular fluorescence intensity by flow cytometry; (C) Fluorescence microscopy images showing the uptake of different formulations by LLC cells; (D) Quantitative analysis of relative cellular fluorescence intensity from fluorescence microscopy images. Scale bar = 50 μm. Data are presented as mean ± standard deviation (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: In Vitro, Fluorescence, Flow Cytometry, Microscopy, Standard Deviation

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: In vitro anti-tumor pharmacodynamic evaluation of biomimetic self-oxygenating M1@CAT-Ce6-Rh2 Lips. (A – B) Representative colony formation images and quantitative analysis of the colony formation rate; (C – D) Representative EDU proliferation images (scale bar: 50 μm) and quantitative analysis of the proliferation rate; (E – F) Representative live/dead staining images (scale bar: 50 μm) and corresponding quantitative analysis; (G – H) Flow cytometric analysis of apoptosis and quantitative determination of the apoptosis rate. Data are presented as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: In Vitro, Staining, Standard Deviation

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: Investigation of the in vitro antitumor mechanism of M1@CAT-Ce6-Rh2 Lips. (A – B) Representative images of total ROS staining and quantitative analysis of fluorescence intensity; (C – D) Intracellular ROS levels detected by flow cytometry and flow quantitative analysis; (E – F) Representative images of 1 O 2 detected by SOSG staining and quantitative analysis of fluorescence intensity; (G – H) Representative images of O 2 − · detected by DHE staining and quantitative analysis of fluorescence intensity; (I – J) Immunofluorescence staining images of SLC7A11 and quantitative analysis of fluorescence intensity; (K – L) Western blot and quantitative analysis of SLC7A11. Data are presented as mean ± standard deviation (n = 3).*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significant difference.

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: In Vitro, Staining, Fluorescence, Flow Cytometry, Immunofluorescence, Western Blot, Standard Deviation

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: Investigation of the relationship between ferroptosis and the antitumor mechanism of M1@CAT-Ce6-Rh2 Lips. (A) Transmission electron microscopy images of mitochondria, → Mitochondrial double membrane; ▲ Mitochondrial cristae; (B) Quantitative analysis of GSH content; (C – D) Western blot and quantitative analysis of GPX4; (E – F) Representative immunofluorescence images and fluorescence intensity analysis of GPX4; (G) Quantitative analysis of MDA content; (H – I) Immunofluorescence staining images and quantitative analysis of 4-HNE; (J – K) C11-BODIPY staining images and quantitative analysis of lipid peroxidation; (L – M) FerroOrange Fe 2+ fluorescence images and quantitative analysis; (N – Q) Mito-Tracker Red (mitochondrial membrane potential, red) and JC-1 staining and corresponding quantitative analysis; (R – S) Staining images and quantitative analysis of mitochondrial permeability transition pore opening. Data are presented as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: Transmission Assay, Electron Microscopy, Membrane, Western Blot, Immunofluorescence, Fluorescence, Staining, Permeability, Standard Deviation

Journal: Materials Today Bio

Article Title: Biomimetic photodynamic nanoparticles exert anti-tumor therapy by inducing ferroptosis in non-small cell lung cancer

doi: 10.1016/j.mtbio.2026.103327

Figure Lengend Snippet: Evaluation of in vivo long-circulating effect and tumor targeting ability of various formulations. (A – B) Observation of the long-circulating effect in mice intravenously injected with different formulations via the tail vein; quantitative analysis of fluorescence signal intensity in blood; (C) Real-time in vivo fluorescence images of tumor-bearing mice detected at demonstrate that nano-formulation, especially M1 macrophage membrane-based modification, can effectively prolong the blood circulation time of Ce6, thereby providing a sufficient time window predetermined time points after intravenous administration of various formulations; (D) Ex vivo fluorescence imaging of major organs and tumors 96 h after intravenous administration (n = 3).

Article Snippet: Equal amounts of protein from M1@CAT-Ce6-Rh2 Lips, M1 membrane, and M1 cells were separated by 10% SDS-PAGE, transferred, and probed with primary antibodies (Servicebio) targeting integrin α4, integrin β1, and β-actin (internal control).

Techniques: In Vivo, Injection, Fluorescence, Formulation, Membrane, Modification, Ex Vivo, Imaging